E85 is an alcohol fuel mixture of 85% ethanol and 15% gasoline, by volume. ethanol derived from crops (bioethanol) is a biofuel.

E85 as a fuel is widely used in Sweden and is becoming increasingly common in the United States, mainly in the Midwest where corn is a major crop and is the primary source material for ethanol fuel production.

E85 is usually used in engines modified to accept higher concentrations of ethanol. Such flexible-fuel engines are designed to run on any mixture of gasoline or ethanol with up to 85% ethanol by volume. The primary differences from non-FFVs is the elimination of bare magnesium, aluminum, and rubber parts in the fuel system, the use of fuel pumps capable of operating with electrically-conductive (ethanol) instead of non-conducting dielectric (gasoline) fuel, specially-coated wear-resistant engine parts, fuel injection control systems having a wider range of pulse widths (for injecting approximately 30% more fuel), the selection of stainless steel fuel lines (sometimes lined with plastic), the selection of stainless steel fuel tanks in place of terne fuel tanks, and, in some cases, the use of acid-neutralizing motor oil. For vehicles with fuel-tank mounted fuel pumps, additional differences to prevent arcing, as well as flame arrestors positioned in the tank's fill pipe, are also sometimes used.